Manufacture of new cardio-active glucosides of bulbus scille



Patented Mar. 21, 1933 stars rArssn WALTER KREIS, 0F BINNINGEN NEAR BASEL, SWITZERLAND, ASSIGNOR TO CHEMICAL V W FQRMERLY SANBGZ, OF BASEL, SEVITZERLAND IVIANTIFACTURE 01 NEW CARDIG-ACTIVE GLUCOSIDES 0F BULBUS SCILLZE No Drawing. Application filed September 8, 1927, Serial No. 218,366, and in Germany August 12, 1927.

It has been found, that the cardio-active glucoside of bulbus Scillze, as prepared according to'the United States Letters Patent No. 1,516,552 and No. 1,579,338, can be separated into two components which are coexisting in the drug. Though thephysical and chemical properties of thesecomponents are distinctly different, theyare so intimately connected-with each other during the preparation of the active principle, that the hitherto known methods of. isolation have al.- ways furnished a mixture.

In a pu lication of Ewins (Journ. of Pharmakology and Experim- Therapeutics vol. III, page 155, 1911) it has already been surmised, that two active principles are coexisting inthe squill, but the author has not characterized them as welldefined substances. According to the method of isolation and especially, in view of the feeble activity of one of the substances prepared by Ewin's, it is evident that these components can only be considered as more or less decomposed products. The great difierence which exists between the substances of Ewins and those obtained according to the present process, by their method of isolation and their properties show that the substances of Ewins cannot be identical to those prepared according tothe present process, which leads to substances hitherto unknown. 7 v

The present process for the separation of the natural cardio-active glucoside of squill is based upon the difference in the solubility of both components inwater and aqueous media, e. mixtures of water with alcohol or methanol. This difference was unknown. It exists even when the components are in the form of their compounds with natural tannin, the so-called tannoids. For distinction purposes I call hereinafter the less soluble component A and the more soluble B. By the aid of a new color-reaction which will be described below, it became possible to dis tinguish that two natural glucosides are coexisting in the squill, whilst the physiological activity, in whichboth components are very similar, could not be used for this purpose. Oomponent Adissolved in acetic anhydride, gives onthe addition of concentrated tannin precipitating acidulated and shaken with, for example,

sulphuric acid a carmine color which imme diately afterwards turns to an emerald-green one. The component B, under the same conditions gives a pure blue color, without showing a redphase at the beginnin As al-.

ready mentioned, the two components adhere obstinately to each other in the drug and also during the isolation of the glucoside. Therefore, it could not be foreseen, that it would be possible to separate the two components A and B and to obtain the pure glucosides in such a simple manner as described in the are present noids.

Tn application of the newly found difference in the solubility of both components present process, even when these components n the form of their so-called tanand the use of the color reaction as acontrol,

has rendered it possible to carry out the process, even with a relatively raw initial material. It has been shown, for example, that the difference in the solubility of the components in water or in aqueous mediums exists even when many impurities are presout, as is the case with an-alcoholic and pre viously purified extract of the drug.

A raw extract can be suitably purified, by CllSSOlVlIIglH methanol and afterwards pre- 'cipitating the principal quantity of the impurities with chloroform, whilst the gIucO- Q B is facilitated by the addition of salt to the watery suspension. The component B remains in solution in tannoid form.

Its aqueous solution is then treated with agents, afterwards chloroform, toremo ve' acid impurities. The

solution is then neutralized and saturated with an easily soluble salt, e. sulphate of ammon a, and the pure glucosideB exhaust vely extracted by shaklng with ethyl acetate. After evaporating the solution in vacuo at a low temperature and after treat- I the following way.

ing the residue with ether, the pure glucoside B remains.

The separation of the components can be carried out in an analogous manner with an initial material, which has been prepared in A raw extract is suspended in water, the solution saturated with salt and the so-called tannoids extracted with ethyl acetate, whereby the inactive im purities remain in the aqueous solution. The ethylacetate solution is then evaporated to dryness and a mixture of the components A and B in the form 01"- tann'oid remains, with which the separation can be eilected by fractional dissolution in water and the pure glucosides can be obtained as described above (see also Example 2). The separation of the components may also be eli'ected by means of fractional precipitation from an organic solvent, e. g. alcohol with water, whereby component A precipitates first or by fractional extraction from an aqueous solution with an organic solvent, e. g. ethyl acetate, whereby component A is first extracted. In any case the separation of the cor ponents is based upon the difference existing in the-solubility of A and B in water or in aqueous media. The carrying out of the process is not limited to the manners described in the examples. A separation of the components A and B, which always to 'xist'in squill, is 'obt ined in all cases, as well as their isolation as free glucosides. The latter substances only exist in nature as so-called tannoids.

The component A, which, as a rule, constitutes of the totality of the natural glucoside of squill, crystallizes from methanol (97%) in the form of quadrangular and hexagonal oblong and clear plates, contain ing 6 of solvent of crystallization, which separates oil completely only in vacuo at 76 C. The substance freed from the solvent of crystallization is a, white powder, which is very diflicultly soluble in water and chloroform, and insoluble in ether. It is rather 'difiicultly soluble in cold methyland ethylalcohol and only rendered more easily soluble by warming. A; 5% solution of the component A in a mixture of parts by volume of ethylalcohol and 25 parts by volume cf water shows a rotary p'cwerfof The elementary analysisgives the following result: 0 82.370 and H==7.6.%.

Tl c glucoside is easily hydrolyzed in aqueous solutions and this hydrolyzation can be quickly completed by the addition of mineral acids.

hand glucose and rhamnose, these products bein obtained in the molecular r0 ortion O I p I he products of hydrolyzation are, on the one hand, an aglucone A, which crystall zes in beautiful columns, on the other 1 1 1. The elementary analysis of the aglucone gives the following result: 0 78377 and H =8.3%. Aglucone A. can be sublimated in vacuo, whereby one molecule of water is split oil". The glucoside, as well as the aglucone, when dissolved in a mixture of parts of acetic anhydride and2 parts of concentrated sulphuric acid, give first a carmine color and immediately afterwards an emerald green color. I

One mg. of the glucoside component A possesses a physiological activity of about 1050 frog doses by onemg. of substance according to the frog standard of Houghton-Straub. The aglucone is less active.

The component B, the isolation of which is claimed by the present process could hitherto not be obtained in a crystalline form; it constitutes a white powder when in a pure state. Contrarily to the component A, it is easily soluble in water and alcohols, difiicultly soluble in chloroform, ether and ethylacetate, but more easily soluble in these solvents than thccomponent A; the component B is dextrorotary. It is more resistant to hydrolysis than A and is only slowly hydrolyzed by dilute mineral acids, whereby a well crystallizing aglucone is also formed, but only with a small yield. Aglucone B crystallizes from methanol in colorless, glossy m long needles. "It is insoluble in water and ether and somewhat more easily soluble in the usual solvents than aglucone A.

The elementary analysis of aglucone B gives the following result: U=73.1%, H 7 .4%'. The aglucone B cannot be sublimated. It sinters at 225 C. and melts under decomposition at 228229 C. A mixture of acetic anhydride and concentrated sulphuric acid (100:2) dissolves both the 'glucoside 7 the aglucone B with a deep blue color, which remains for some length of time. I

' One mg. of the glucoside component B possesses a physiological. activity of at least and 15004600 frog doses (frog standard of Houghton-Straub) 4 The following examples illustrate the improved process.

7 Eccamplc 1 150 g. of a raw extract, obtained by eX- traction of carefully dried and powdered uills with alcohol of 95% strength at a low temperature and evaporating the solventin vacuo at a low temperature to dryness,

are dissolved "in. 150 com. of cold methanol of 99% and filtered. inlltGlWVfll'tlS litres of chloroform arc poured into the filtrate While stirring, whereby the principal quantity of the impurities are precipitated. The liquid, after having been allowed to stand over night, is decanted, filtered and evaporated in vacuo to dryness.

'50 gQof the dried residueare stirred with a small quantity of cold water to a homoextracted with ethylacetate.

which is obtained, by evaporating the ethyl geneous pulp,'which is then diluted with ponent-B and generally "showing a weakly acid reaction, is carefully neutralized with diluted caustic soda for instance. The'solution is then shaken with asmall portion of an insoluble tannin precipitating agent, e. g. lead hydroxide, until the latter remains white. Acidadmixtures are removed by the addition of l ccm. of 2 n HQSO. per litre of liquid and exhaustive extraction with chlroform. The watery solution is again neutralized with soda and saturated with sulphate of ammonia and the glucoside B then exhaustively extracted with ethyl acetate. The ethyl acetate solution is evaporated in vacuo at a low temperature until it is almost dry. Finally the residue is treated with ether and filtered. The properties of the dried glucoside B, thus obtained, correspond to those mentioned above.

Example 2 and the extract evaporated in vacuo at a low temperature to dryness. The residue was dissolved in water, 100 g. of common salt were added per litre and the solution exhaustively The product acetate in vacuo to dryness, consists of a mixture of the components A and B in the form of their so-called tannoids. Of this mixture g. are stirred in a mortar with a little wa ter to a homogeneous paste, and this is then further diluted by addition of water, whilst continually stirring, whereby the diflicultly soluble component A generally precipitates in a fiocculent state. If lumps are formed, they should be granulated by the addition of a little water. The total quantity of water used should not exceed one litre. The precipitation'of the component A is completedby the addition of 25 com. of a saturated com mon salt solution. The mass is left to stand for a short time, and the residue containing the tannoid A is separated by filtration. The watery solution of the component B as obtained after separation of the tannoid component' A is, after careful neutralization, shaken with small quantities of an'insoluble tannin precipitating agent, as indicated'in Example 1, until the latter remains white.

Some impurities still remaining inthe last filtrate are removed by acidulating with 1 com. of 2 N sulphuric acid per litrean'd thoroughly shaking with some chloroform. The watery solution is again neutralized and carefully evaporated in vacuo to dryness. Theglucoside B may be obtained in a pure state by extracting the' residue with absolute alcohol. After evaporating the solvent in vacuo at a low temperature, the glucosideB remains, possessingthe above described properties. c

What I claim is: c 1 '1. A process for the isolation of aneasi'ly soluble cardio-active glucoside from bulbus Scillae, comprising the steps of dissolvinga raw alcoholic extract from bulbus Scillm in anv aliphatic alcohol, selected from a. class comprising .methanol and. ethanol, treating this solution with chloroform in order to pre-' cipitate the inactive. impurities, filtering the solution, evaporating same in vacuo at a'low temperature, extracting the residue thus ob- I order to separate the water insoluble portion, treating the obtained aqueous. extract with tannin precipitating substances, separatingthe solution from the .precipitatethus obtained, extracting the easily soluble glucoside from the solution byv means of a-neutral aliphatic organic liquid from the class of chloroform and ethylacetate, and isolating the glucoside in dry form from its solution in the organic solvent by evaporatingthe'solvent in vacuo. v a

2. A process for the isolation of an easily soluble cardio-active glucoside from bulbus Scillae, comprising the steps of dissolving-a raw alcoholicextract from bulbus Scillae in .an aliphatic alcohol selected from'a class comprising methanol and ethanol,treating this solution. with chloroform in order .to

precipitate the inactive impurities, filtering the solution, evaporating same in vacuo at a low temperature,extracting the residue thus obtained which consists of a mixture of tannoids of. the active glucosides, with water in presence of easily water soluble salts, in order p to separate the water-insoluble, portion, treating the obtained aqueous extract side from the solution by means of a neutral aliphatic organic liquid fromthe class of chloroform and ethylacetate, and isolating the glucoside in dry form from its solution inthe organic solvent by evaporating the solvent-in vacuo.

.3. A process for the isolation of an easily soluble cardio-active glucoside from bulbus Scillae, comprising the steps of dissolvinga raw. alcoholic extract from bulbus Scillae in an aliphatic alcohol selected from a class comprising methanol and ethanol, treating this solution with chloroform, in order to precipitate the inactive impurities, filtering the solution, evaporating the same invacuo at a low temperature, extracting the residue thus obtained, which consists of a mixture of tannoids of the activeglucosides, with water in presence of easily water soluble salts, in order to separate the water-insoluble portion,--treating the obtained aqueous extractv with tannin precipitating substances, separating the solution from the precipitate thus obtained, extracting the easily soluble glucoside from the solution by means of a neutral-aliphatic organic liquid from the class of chloroform and ethylacetate inpresence of a larger amount of easily water solu ble salts, and isolating the glucoside in dry form from its solution in the organic solvent by evaporating the solvent in vacuo.

. 4. A process for the isolation of an easily soluble cardio-activeglucoside from bulbus Scillae, comprising the steps of dissolving a raw alcoholic extract from bulbus Scillae in isolating the glucoside in dry form from its solution in the organic solvent by evaporating the solvent in vacuo. I

6. As a new article of manufacture the herein described cardio-active substance of bulbus Scillze, consisting of a body in form of free glucoside, being a white powder easily soluble in water, methyland ethylalcohol, diificultly soluble in chloroform, ether and ethylacetate, being dextrorotary, giving on hydrolyzation sugar and an aglucone, the latter crystallizing from methanol in form of colorless, long needles, the aglucone containing 73.1% of carbon and 7.4% of hydrogen,both the glucoside and the aglucone giving with acetic anhydride and sulphuric acid a deep blue color, 1 mg; of .the glucoside possessing a physiological activity ofatleast 1600 frog doses, according to; the frog standardiof Houghton-Straub. V

In witness whereofI have hereunto signed my name this 29th day of August 1927.

WALTER KREIS.

an aliphatic alcohol selected from a class comprising methanol and ethanol, treating this solution with chloroform in order to a tannoids of the active glucosides, with Water in presence of easily water soluble salts, in order to separate the water-insoluble portion,

water insoluble tannin precipitating substances, separating the solution from the pretreating the obtained aqueous extract with cipitate thus obtained, extractingthe easily V soluble glucoside from the solution by means of a'neutral aliphatic organicliquid from the class of chloroform and 'ethylacetate in .7

presence of a largeramount of easily water soluble salts, and isolating the glucoside in dry formfrom its solution in the organic solvent by evaporating the solvent in vacuo.

5. A process for the isolation of an easilysoluble cardio-active'glucoside from bulbus Scillae, comprising the steps of extracting a purified extract from bulbus Scillae, which consists of a mixture of tannoids of the active glucosides, with water in presence of easily water soluble salts, in order to separate the water-insoluble portion, treating the obtained aqueous extract with water-insoluble tannin 6b precipitating substances, separating the solution from thewprecipitate thus obtained, extracting the easily soluble glucoside from organic liquid from the class of chloroform the solutionby means of a neutral aliphatic 

